Phyllanthus emblica Buffer Extract [PEBE] and Citrus reticulata, Rutaceae Buffer Extract [CRRBE] characterization and its role on biomedical application are the scope of this study. In preliminary characterization PEBE shows the presence of flavonoids, alkaloids, proteins and phenolic compounds. In the other hand CRRBE shows the presence of terpenoids, saponins, proteins, alkaloids and phenolic compounds. To verify the presence of phytoconstituent, both PEBE and CRRBE were subjected to HPLC and GC-MS analysis. Interestingly, PEBE elutes 5 and 7 peaks in HPLC and GC-MS respectively. Whereas, CRRBE elutes 3 and 9 peaks in HPLC and GC-MS respectively. In addition, both PEBE and CRRBE found to be presence of several minerals such as aluminium, boron, copper, iron and etc. Furthermore, PEBE and CRRBE exhibit anti-microbial activity by inhibiting the growth of pathogenic organisms in respective media. Moreover, both PEBE and CRRBE exhibit non-toxic property as it is unable to cleave packed RBC.